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KMID : 0644120060190010069
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Journal of Oriental Gynecology
2006 Volume.19 No. 1 p.69 ~ p.80
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Rhizoma Rehmanniae induced Apoptosis in Human Cervical Carcinoma HeLa Cells
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Kim Ju-Yeoun
Jo Ok-Hyun
Choe Chang-Min
Cho Han-Baek
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Abstract
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Purpose : This study is to evaluate the synergistic cytotoxicity of Rhizoma Rehmanniae(RR), in adriamycin-treated HeLa human cervical carcinoma cells.
Methods : We culture HeLa cell which is human metrocarcinoma cell in D-MEM included 10% fetal bovine serum(Hyclone Laboratories) below 37^{circ}C, 5% CO_2. Then we observed apoptosis of log phage cell which is changed cultivation liquid 24 Hours periodically.
Results : The combination of RR and adriamycin synergistically augmented the cytotoxicity of HeLa cells. The apoptotic cell death was accompanied by the activation of caspase-3 and -8 as well as cleavage of poly(ADP- ribose) polymerase (PARP) in HeLa cells. The co-treatment of RR with adriamycin didn’’t have any effect on either the expression of Bcl-2 or that of Bax. Interestingly, a synergistic increase in apoptosis by the combination of two drugs was accompanied by the enhancement of Pas and Fas ligand (FasL) expression in HeLa cells. Taken together, the combination of RR and adriamycin significantly augmented the apoptotic cytotoxicity of Fas-positive cells, such as HeLa cells. The pathway is not involved in mitochondria-dependent pathway.
Conclusion : RR induces apoptosis in HeLa cells via p38 MAPK activation.
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KEYWORD
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Rhizoma Rehmanniae (RR), caspase, Fas/FasL, apoptosis, HeLa cells
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